AgronomyEN

Our lab：

2017-present: Our lab use Gene Editing or Base Editing technology to create new crop genes’ functions. For example: by modifying the 177th amino acid proline of rice EPSPS gene to serine (DNA modification replaced by CCA with TCA), It retains the functions of EPSPS gene and is resistant to the herbicide Glyphosate.

2015-2017：Cooperated with Hualien District Agricultural Research and Extension Station to study the active ingredient of Chinese herbal medicine Angelica dahurica.

2000-2017：Used tobacco, tomato and rice as materials to study plant transposon

1. Studied the mechanism and efficiency of transposon
2. Created new transposon to induce the formation of a promotor to drive the transposase into inducible transposon
3. Applied the above-mentioned inducible transposons as biological mutagens to create crop mutants and provide functional gene research.
4. Modified rice EPSPS gene to herbicide-resistant Glyphosate, which was used as a trans-gene marker, and matched the above-mentioned inducible transposon (constructed in the iron of the EPSPS gene) to become a genetically modified crop and the tool for selecting marker was removed after the transgene was completed
5. According to the above concept, we can construct an inducible transposon in the intron of its own transposase gene to become a new transposon (jumping gene).
6. The above research results also found that when the transposon is located in the intron of a gene, it can provide new transcriptome and increase evolutionary competitiveness without destroying the gene. Therefore, during the evolution process, the transposon not only jump into a gene and break it, but also improve the gene’s functions. This process is called exoniaztion.
7. According to point 6, using bioinformatics tools to thoroughly study maize transposon exoniaztion has potential contribution to evolution, including transcriptome diversity and proteome function analysis. It was found that two maize transposons with close relatives provide different proteome functional diversity.
   
   Please refer to the following link for the early popular science discourse
   
   亮不亮？有關係！---螢火蟲的冷光基因在轉殖植物上的應用
   
   利用轉位子在蕃茄中大量釣取基因及啟動子
   
   跳不跳？有關係！---轉位子與植物基因篩選
   
   在轉殖水稻中利用R/RS特定位置之重組及Ac轉位子來誘導基因體序列缺失的系統
   
   誤解小辭典
   
   轉位子在跨物種上的研究與應用
   
   植物化學可誘導基因表現系統

Academic Degress

Experience

1. US Patent（2013），Title：Termination of Transgene Expression via Transposon-Mediated Break。Number：US 8,524,979 B。Duration：~August 2033。

2. US Patent（2006），Title：Live vaccines for allergy treatment。Number：US7060687B2。Duration：~ May 2026 。

3. Domestic Patent（2005），Title：可誘發之單一成分植物基因標記物。 Number：I233332號。Duratin：~ January 2021

4. US Patent（2002），Title：Inducible One-Component Plant Gene tagging。Number：US 6369297B1。Duration：~ May 2020。